Soil microbial community analysis using terminal restriction fragment length polymorphisms

摘要: Terminal restriction fragment length polymorphism (T-RFLP) analysis is a polymerase chain reaction (PCR)-fingerprinting method that commonly used for comparative microbial community analysis. The can be to analyze communities of bacteria, archaea, fungi, other phylogenetic groups or subgroups, as well functional genes. rapid, highly reproducible, and often yields higher number operational taxonomic units than other, PCR-fingerprinting methods. Sizing terminal fragments (T-RFs) now done using capillary sequencing technology allowing samples contained in 96- 384-well plates sized an overnight run. Many multivariate statistical approaches have been interpret compare T-RFLP fingerprints derived from different communities. Detrended correspondence the additive main effects with multiplicative interaction model are particularly useful revealing trends data. Due biases inherent method, linking size T-RFs complex existing sequence databases infer their position not very robust. This approach has successfully, however, identify follow dynamics members within simple successfully composition soil, water, marine, lacustrine sediments, biofilms, feces, on plant tissues, digestive tracts insects mammals. user-friendly molecular adding significant information studies populations many environments.