Protein kinase D distribution in normal human epidermis, basal cell carcinoma and psoriasis.

摘要: Summary Background  Keratinocytes undergo a defined programme of proliferation and differentiation during normal stratification the epidermis. Anomalies in signalling pathways controlling this process probably contribute to pathogenesis hyperproliferative dermatological diseases, including psoriasis basal cell carcinoma (BCC). We have previously proposed that protein kinase D (PKD) is proproliferative enzyme keratinocytes speculated abnormalities its levels or regulation may disorders skin. Objectives  To determine if human skin are characterized by abnormal expression distribution PKD, epidermis was compared with BCC uninvolved involved psoriatic epidermis. Methods  examine expression, immunohistochemical analysis samples Western blotting neoplastic mouse performed. cells using phosphospecific PKD antibody allowed estimation activation status. Results  Normal demonstrated predominant stratum basalis, proliferative epidermal compartment, decreased relative throughout suprabasal strata. Uninvolved showed similar pattern, but contrast, lesions diffuse staining all The majority BCCs examined significant and, those biopsies which could be compared, elevated status were also increased keratinocyte line. Conclusions  misdistributed disorders, psoriasis, as well keratinocytes. speculate exerts and/or antidifferentiative effects epidermis, anomalous precipitating sustaining disease psoriasis.