Specific prebiotic oligosaccharides modulate the early phase of a murine vaccination response.

摘要: Abstract The immune-modulatory effect of specific prebiotic oligosaccharides was shown in previous preclinical and clinical studies. To enhance the understanding this effect, kinetic aspects immune modulation correlation between microbiological immunological parameters were investigated a murine vaccination model. C57BL/6 mice supplemented with short-chain galactooligosaccharides long-chain fructooligosaccharides (ratio 9:1; Immunofortis™) combination pectin-derived acidic oligosaccharides. timing supplementation varied around primary (day 0) secondary 21) vaccinations. Supplementation before necessary to increase delayed-type hypersensitivity responses (DTH) significantly at day 30. after 8 did not affect DTH response 30, indicating that occurred during early phase. Therefore, analysis performed shortened experiment focus on At 9 post-priming, percentages cecal lactobacilli correlated (p = 0.01). Furthermore, results suggested yet unidentified factors may play role. Additional intestinal Peyer's patch major lymphocyte populations show effects supplementation. In conclusion, oligosaccharide mixture exert its phase response. are consistent role microbiota possibly other oligosaccharide-induced modulation. demonstrate it is critical consider order study their interaction meaningful way.