Normalization of Tumor Microenvironment by Neem Leaf Glycoprotein Potentiates Effector T Cell Functions and Therapeutically Intervenes in the Growth of Mouse Sarcoma

摘要: We have observed restriction of the murine sarcoma growth by therapeutic intervention neem leaf glycoprotein (NLGP). In order to evaluate mechanism tumor restriction, here, we analyzed microenvironment (TME) from bearing mice with NLGP therapy (NLGP-TME, in comparison PBS-TME). Analysis cytokine milieu within TME revealed IL-10, TGFβ, IL-6 rich type 2 characters was switched 1 dominance IFNγ secretion NLGP-TME. Proportion CD8+ T cells increased NLGP-TME and these were protected TME-induced anergy NLGP, as indicated higher expression pNFAT inhibit related downstream signaling. Moreover, low FasR+ cell population denotes prevention activation induced death. Using CFSE a probe, better migration noted treated than PBS cohort. isolated exhibited greater cytotoxicity vitro show molecules, perforin granzyme B. Adoptive transfer exposed cells, but not PBS-TME mice, is able significantly vivo. Such inhibition when depleted for cells. Accumulated evidences strongly suggest mediated normalization allows perform optimally growth.