Extraneuronal effects of 6-hydroxydopamine. Tissue culture studies on adrenocortical cells of rats.

摘要: The effects of various concentrations 6-hydroxydopamine (6-OHDA) on rat adrenocortical cells in tissue culture were studied with phase contrast and electron microscopy. With 40 mg/l 6-OHDA the first signs alteration as revealed by microcinematography appeared isolated cortical early 15 min after addition drug. There was a cessation movement cell organelles an immobilisation membrane undulations followed development dark inclusion bodies. underwent increasing shrinkage collapsed 1 1/2 h. Chromaffin not affected until 45 exposure to drug neurons most resistant population. However 6 h application dead. applied different doses adrenal explants did alter sequence events. Ultrastructurally cortex damage alone two lines: they either showed lytic changes or developed types dense bodies before reaching stage. Treatment 5-or buffered 2% glyoxylic acid heat produce fluorescence within cells. Microspectrofluorimetry amine models noradrenaline, 5- that neither 5-nor are capable form fluorophore acid.