Gene synthesis by circular assembly amplification

作者: Duhee Bang , George M Church

DOI: 10.1038/NMETH1136

关键词:

摘要: Compositions and methods for synthesizing polynucleotides, reducing errors during polynucleotide synthesis, correcting mismatches in synthetic sequences, selecting correctly assembled sequences are provided.

参考文章(16)
Hugo G Menzella, Ralph Reid, John R Carney, Sunil S Chandran, Sarah J Reisinger, Kedar G Patel, David A Hopwood, Daniel V Santi, Combinatorial polyketide biosynthesis by de novo design and rearrangement of modular polyketide synthase genes Nature Biotechnology. ,vol. 23, pp. 1171- 1176 ,(2005) , 10.1038/NBT1128
Jeffrey S. Han, Jef D. Boeke, A highly active synthetic mammalian retrotransposon. Nature. ,vol. 429, pp. 314- 318 ,(2004) , 10.1038/NATURE02535
H. O. Smith, C. A. Hutchison, C. Pfannkoch, J. C. Venter, Generating a synthetic genome by whole genome assembly: φX174 bacteriophage from synthetic oligonucleotides Proceedings of the National Academy of Sciences of the United States of America. ,vol. 100, pp. 15440- 15445 ,(2003) , 10.1073/PNAS.2237126100
Jingdong Tian, Hui Gong, Nijing Sheng, Xiaochuan Zhou, Erdogan Gulari, Xiaolian Gao, George Church, Accurate multiplex gene synthesis from programmable DNA microchips Nature. ,vol. 432, pp. 1050- 1054 ,(2004) , 10.1038/NATURE03151
S. J. Kodumal, K. G. Patel, R. Reid, H. G. Menzella, M. Welch, D. V. Santi, Total synthesis of long DNA sequences: Synthesis of a contiguous 32-kb polyketide synthase gene cluster Proceedings of the National Academy of Sciences of the United States of America. ,vol. 101, pp. 15573- 15578 ,(2004) , 10.1073/PNAS.0406911101
Ai-Sheng Xiong, Quan-Hong Yao, Ri-He Peng, Xian Li, Hui-Qin Fan, Zong-Ming Cheng, Yi Li, A simple, rapid, high-fidelity and cost-effective PCR-based two-step DNA synthesis method for long gene sequences Nucleic Acids Research. ,vol. 32, ,(2004) , 10.1093/NAR/GNH094
Peter A Carr, Jason S Park, Yoon-Jae Lee, Tiffany Yu, Shuguang Zhang, Joseph M Jacobson, Protein-mediated error correction for de novo DNA synthesis Nucleic Acids Research. ,vol. 32, ,(2004) , 10.1093/NAR/GNH160
L. Young, Two‐step total gene synthesis method Nucleic Acids Research. ,vol. 32, ,(2004) , 10.1093/NAR/GNH058