Comparative assessment of genetic and epigenetic variation among regenerants of potato (Solanum tuberosum) derived from long-term nodal tissue-culture and cell selection
作者： Alison L. Dann , Calum R. Wilson
DOI: 10.1007/S00299-010-0983-9
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摘要: Three long-term nodal tissued cultured Russet Burbank potato clones and nine thaxtomin A-treated regenerant lines, derived from the were assessed for genetic epigenetic (in form of DNA methylation) differences by AFLP MSAP. The treated lines originally selected superior resistance to common scab disease acceptable tuber yield in pot field trials. long-term, tissue culture clone exhibited (8.75–15.63% polymorphisms) (12.56–26.13% between them may represent a stress response induced normal plant growth disruption. much higher significant (p < 0.05) (2–29.38%) (45.22–51.76%) polymorphisms than parent clones. Methylation-sensitive mutations accumulated within are significantly correlated resistance. However, linking phenotypic that could be benefit growers, single gene sequence tetraploid such as would extremely difficult since it is assumed many desirable traits under polygenic control.
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