Monoclonal antibodies against the human epidermal growth factor receptor from A431 cells. Isolation, characterization, and use in the purification of active epidermal growth factor receptor.

摘要: A431 cells have been used as an immunogen for generating monoclonal antibodies against the epidermal growth factor (EGF) receptor. Two immunoglobulin M and eight G3 anti-EGF receptor were cloned. All ten immunoprecipitated biosynthetically labeled mature cell EGF able to recognize in Western blotting. However, none of precursor polypeptides receptor, neither did they receptors from human foreskin fibroblasts, placenta, nor a human-mouse hybrid expressing The found bind glycolipids it was shown that determinant involved blood group A antigen. It appears this is present on both but not expressed other tested. One raised immunoaffinity purification procedure took advantage carbohydrate nature antigenic by employing sugar-specific elution. mild conditions permitted (70-80% pure) possessed intrinsic EGF-stimulated tyrosine kinase activity with specific about 20 nmol/min/mg.