Reproductive toxicity of di-n-butylphthalate in a continuous breeding protocol in Sprague-Dawley rats.

摘要: The phthalate ester di-n-butylphthalate (DBP) is used extensively in the manufacture of plastics; its reproductive toxicity was tested rats by National Toxicology Program's Reproductive Assessment Continuous Breeding protocol. Levels 0.1, 0.5, and 1.0% DBP diet were selected, this dosing design yielded average daily intakes 52, 256, 509 mg/kg for males 80, 385, 794 females, respectively. consumption F0 reduced total number live pups per litter all treated groups 8-17% pup weights 0.5% dose < 13%. In tests to determine affected sex, offspring unchanged, but from females significantly decreased unchanged. At necropsy, high-dose had a 14% reduction body weight, both sexes approximately 10-15% increased kidney liver weight ratios compared controls. Sperm parameters estrous cyclicity not affected. F1 mating trial, indices mating, pregnancy, fertility group sharply (one delivered out 20 cohabited pairs), concomitant with 13% decrease dam weight. Live F2 6-8% lower groups. necropsy results revealed that epididymal sperm counts testicular spermatid head group. Histopathologic investigation showed 8 10 consuming degenerated seminiferous tubules 5 underdeveloped or otherwise defective epididymides. No ovarian uterine lesions observed. conclusion, study reproductive/developmental toxicant Sprague-Dawley exposed as adults during development; it also indicates adverse effects on second generation greater than first generation.