Conditional Expression of RNA Polymerase II in Mammalian Cells
作者： Mark Meininghaus , Rob D. Chapman , Manuela Horndasch , Dirk Eick
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摘要: The carboxyl-terminal domain (CTD) of the large subunit mammalian RNA polymerase II contains 52 repeats a heptapeptide that is target variety kinases. hyperphosphorylated CTD recruits important factors for mRNA capping, splicing, and 3′-processing. role transcription process in vivo, however, not yet clear. We have conditionally expressed an α-amanitin-resistant with almost entirely deleted (LS*Δ5) B-cells. These cells defect global cellular genes presence α-amanitin. Moreover, pol harboring LS*Δ5 failed to transcribe up promoter-proximal pause sites thehsp70A c-fos gene promoters. results indicate already required steps occur before pausing maturation mRNA.
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