Ionizing radiation enhances matrix metalloproteinase-2 production in human lung epithelial cells.
作者： Jun Araya , Muneharu Maruyama , Kazuhiko Sassa , Tadashi Fujita , Ryuji Hayashi
DOI: 10.1152/AJPLUNG.2001.280.1.L30
关键词:
摘要: Radiation pneumonitis is a major complication of radiation therapy. However, the detailed cellular mechanisms have not been clearly defined. Based on recognition that basement membrane disruption occurs in acute lung injury and matrix metalloproteinase (MMP)-2 can degrade type IV collagen, one components membrane, we hypothesized ionizing would modulate MMP-2 production human epithelial cells. To evaluate this, modulation with irradiation was investigated normal bronchial cells as well A549 We measured activity conditioned medium zymography mRNA level RT-PCR. Both these constitutively expressed 72-kDa gelatinolytic activity, corresponding to MMP-2, exposure increased this activity. Consistent data zymography, mRNA. This radiation-induced increase expression mediated via p53 because antisense oligonucleotide abolished accumulation after These results indicate by involved injury.
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