A Comparative Study of the Metabolic and Skeletal Response of C57BL/6J and C57BL/6N Mice in a Diet-Induced Model of Type 2 Diabetes

摘要: Type 2 diabetes mellitus (T2DM) represents a complex clinical scenario of altered energy metabolism and increased fracture incidence. The C57BL/6 mouse model diet-induced obesity has been used to study the mechanisms by which glucose homeostasis affects bone mass quality, but genetic variations in substrains may have confounded data interpretation. This investigated long-term metabolic skeletal consequences two commonly high fat (HF) diet. Male C57BL/6J, C57BL/6N, negative control strain, C3H/HeJ, mice were fed or HF diet for 24 wks. C57BL/6N on demonstrated an increase plasma insulin blood as early 4 wk, whereas these responses delayed C57BL/6J mice. exhibited more severe hepatic steatosis inflammation. Only lost significant trabecular response C3H/HeJ protected from loss. show that differ their when These substrain differences should be considered designing experiments are likely implications interpretation reproducibility.