Single microtubules from squid axoplasm support bidirectional movement of organelles

摘要: Abstract Single filaments, dissociated from the extruded axoplasm of squid giant axon and visualized by videoenhanced differential interference contrast microscopy, transport organelles bidirectionally. Organelles moving in same or opposite directions along filament can pass each other without colliding, indicating that has several tracks for organelle movement. In order to characterize movements were first examined video then filaments electron microscopy after rapid-freezing, freeze-drying, rotaryshadowing. Transport supported bidirectional movement are 22 nm 27 diameter have a substructure indicative single microtubule. Immunofluorescence showed virtually all contain tubulin. These results show microtubules serve as substratum movement, suggest an interaction between is basis fast axonal transport.