Celecoxib Combined with Diacerein Effectively Alleviates Osteoarthritis in Rats via Regulating JNK and p38MAPK Signaling Pathways

摘要: Osteoarthritis (OA) has long been a difficult to overcome joint disease for medical workers. However, there is still lack of effective treatments OA. In the present study, we aimed evaluate treatment effect celecoxib (CLX) combined with diacerein (DC) on OA and delineate underlying molecular mechanism. The model was established by using rats, rats were treated either CLX alone, DC DC. results showed that, as compared single or DC, markedly attenuated inhibited levels inflammatory mediators interleukin-1β nitric oxide, improved bone cartilage metabolism, suppressed chondrocyte apoptosis. Most importantly, significantly inactivated c-Jun N-terminal kinases (JNK) signaling pathway inhibition MEKK1 MKK7, detected Western blot analysis. Furthermore, protein expression downstream genes JNK, including activating-transcription factor (Atf-2), matrix metalloproteinase-13 (MMP-13), cyclooxygenase (COX-2), also treatments. effectively inhibits p38 mitogen-activated kinase nuclear factor-κB pathways. Taken together, our study suggests that satisfactory effects via stronger inhibitory pathway.