Association of the Bacillus subtilis Chromosome with the Cell Membrane: Resolution of Free and Bound Deoxyribonucleic Acid on Renografin Gradients
作者： Robert D. Ivarie , Jacques J. Pène
DOI: 10.1128/JB.104.2.839-850.1970
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摘要: Linear density gradients of Renografin have resolved two components bacterial deoxyribonucleic acid (DNA) in sheared lysates. Component 1, at equilibrium after 5 hr centrifugation, is enriched for newly synthesized DNA and markers near the origin terminus replication. It contains 5% total cellular protein, 25% phospholipids, 30 to 50% DNA, 4 11% unstable ribonucleic (RNA), RNA polymerase, low amounts polymerase. The material sensitive Pronase Sarkosyl. In unsheared lysates, all forms a band this position. Shearing lysate generates slow-sedimenting fraction (component 2) which more uniformly labeled than DNA. These observations suggest that replicating possibly replication are associated with membrane. amount component 1 an estimate number chromosomal fragments other parts chromosome
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