Murine blood banking: characterization and comparisons to human blood.

摘要: Blood transfusion remains an essential treatment of acute anemia. Current storage processes allow the efficient administration blood products. Erythrocytes undergo morphological and biochemical changes during that may affect outcomes after transfusion. A reliable small-animal model would be ideal to examine effects stored products The objective this study was characterize murine erythrocytes for future application animal models samples were collected from male mice human volunteers, separated into components, stored. At intervals, analysis performed. Lactate, potassium, hemoglobin, hemolysis determined, cell morphology evaluated with light microscopy. Murine packed red cells (pRBCs) aged more rapidly than samples. pRBCs exhibited higher lactate levels (34.9 ± 1.3 mmol/L vs. 18.1 1.0 mmol/L, mouse human) severe acidosis as indicated by pH (6.56 0.02 6.79 0.04, human). hemolyzed earlier (11.2 3.7 g 0.7 0.3 g, 21 days storage) pRBCs. Corpuscular consistent lesions appeared in compared Compared pRBCs, exhibit similar but accelerated aging under standard conditions. Characterization lesion will components investigations anemia experimental models.