Overexpression of the EGF Receptor and p53 Mutations are Mutually Exclusive in the Evolution of Primary and Secondary Glioblastomas

摘要: Glioblastoma multiforme, the most malignant human brain tumor, may develop de novo (primary glioblastoma) or through progression from low-grade anaplastic astrocytoma (secondary glioblastoma). We present further evidence that primary and secondary glioblastomas constitute distinct disease entities which acquisition of different genetic alterations. analyzed p53 mutations, protein accumulation epidermal growth factor receptor (EGFR) overexpression in 49 biopsies classified as glioblastoma according to clinical histopathologic criteria. Patients with were selected on basis a history less than 3 months features at first biopsy (19 cases; mean age, 55 years). The diagnosis required least two well histologic low grade (30 39 DNA sequence analysis showed mutations rare (11%) while had high incidence (67%), 90% already biopsy. (nuclear immunoreactivity PAb 1801) was also lower (37%) (97%). In contrast, for EGF prevailed (63%) but (10%). Only one out EGFR mutation. These data indicate tumor suppressor gene are mutually exclusive events defining pathways evolution common phenotypic endpoint.