Extended Electrical Model for Impedance Characterization of Cultured HeLa Cells in Non-Confluent State Using ECIS Electrodes

摘要: Electric cell substrate impedance sensing has been widely used as a label free quantitative platform to study various biological processes and it is extremely essential extract the parameters like variation of spacing, changing projected area on electrode approximate cluster size during non-confluent state understand mechanism proliferation cells. The distributed analytical models developed so far these are applicable only under conditions when cells have become confluent. There some lumped electrical which reported for but they do not provide correct estimate spacing growth. In this paper we develop extended characterize spectroscopy behavior cultured HeLa in 200 Hz 1 MHz range using eight well ECIS electrodes state. model introduces pseudo regularity arrangement average ensemble significant parameters. extracted from after 10 hours, 20 30 hours growth compared with circuit observed fit experimental data seven times improved quality factor. Further, radius at three different instants time found match closely microscopic observation contrast model.