First Insights into the Genetic Diversity of the Pinewood Nematode in Its Native Area Using New Polymorphic Microsatellite Loci

摘要: The pinewood nematode, Bursaphelenchus xylophilus, native to North America, is the causative agent of pine wilt disease and among most important invasive forest pests in East-Asian countries, such as Japan China. Since 1999, it has been found Europe Iberian Peninsula, where also causes significant damage. In a previous study, 94 pairs microsatellite primers have identified silico nematode genome. present specific PCR amplifications polymorphism tests validate these loci were performed 17 that suitable for routine analysis B. xylophilus genetic diversity selected. markers was evaluated on nematodes from four field origins one laboratory collection strain, all originate area. number alleles expected heterozygosity varied between 2 11 0.039 0.777, respectively. First insights into population structure obtained using clustering multivariate methods genotypes samples. results showed spatially structured at scale tree probably larger scales. role dispersal by insect vector versus human activities shaping this discussed.