Alteration of numerical chromosomal aberrations during progression of colorectal tumors revealed by a combined fluorescence in situ hybridization and DNA ploidy analysis of intratumoral heterogeneity

摘要: Abstract To trace the sequence of numerical chromosomal aberrations during tumor progression colorectal tumors, we studied intratumoral heterogeneity copy number by a combined fluorescence in situ hybridization (FISH) and ploidy analysis. We used six formalin-fixed paraffin-embedded tumors which mucosal lesions were preserved. Nuclear suspensions made from tissues that scraped several small regions 100 μm thick sections. Copy chromosomes 1, 7, 17, 18 examined FISH with centromeric repetitive probes. DNA was monitored cytofluorometry, correlated to on smear slides identical nuclear suspension each portion. All included DNA-diploid mucosa, where cancer cells commonly showed monosomy and/or trisomy 7. These changes may be quite common early events before occurrence DNA-aneuploidy development tumors. Three out 6 neartetraploid (3.6–4.1C) deeper invasive regions. Chromosomal constitution DNA-aneuploid suggested derive through duplication or without additional loss gain chromosomes.