Sex-specific eNOS activity and function in human endothelial cells.

摘要: Clinical and epidemiological data show that biological sex is one of the major determinants for development progression cardiovascular disease (CVD). Impaired endothelial function, characterized by an imbalance in Nitric Oxide Synthase (eNOS) activity, precedes accelerates CVD. However, whether there any sexual dimorphism eNOS activity function cells (ECs) still unknown. Here, independently studying human male female ECs, we found ECs expressed higher mRNA protein levels both vitro ex vivo. The increased expression was associated to enzymatic nitric oxide production. Pharmacological genetic inhibition affected migratory properties only ECs. In angiogenesis experiments confirmed sprouting mostly relied on eNOS-dependent migration At variance, capillary outgrowth from independent but required cell proliferation. this study, sex-specific differences EC expression, eNOS. This intrinsic should be further evaluated achieve more effective precise strategies prevention therapy diseases impaired such as CVD pathological angiogenesis.