Orientation-dependent interaction between Drosophila insulators is a property of this class of regulatory elements

摘要: Insulators are defined as a class of regulatory elements that delimit independent transcriptional domains within eukaryotic genomes. According to previous data, an interaction (pairing) between some Drosophila insulators can support distant activation promoter by enhancer. Here, we have demonstrated pairs well-studied such scs-scs, scs'-scs', 1A2-1A2 and Wari-Wari the white yeast GAL4 activator in orientation-dependent manner. The same is true for efficiency enhancer stimulates expression eyes. In all insulator tested, stimulation gene was stronger when were inserted eye or opposite orientations relative each other. As shown previously, Zw5, Su(Hw) dCTCF proteins required functioning different do not interact with strong functional interactions been revealed DNA fragments containing binding sites either Zw5 protein but heterologous [Zw5-Su(Hw), dCTCF-Su(Hw), dCTCF-Zw5]. These results suggest selective elements.