Rapid multiparameter analysis of cell stimulation in mixed lymphocyte culture reactions.
作者： F Traganos , A J Gorski , Z Darzynkiewicz , T Sharpless , M R Melamed
DOI: 10.1177/25.7.142787
关键词:
摘要: A flow-cytofluorometric method, based on the differential stability of deoxyribonucleic acid versus ribonucleic with metachromatic dye, acridine orange, simultaneously measures following parameters stimulation in mixed lymphocyte cultures: (a) number nonstimulated cells; (b) total stimulated lymphocytes; (c) lymphocytes G1, S and G2 + M phases cell cycle; (d) macrophages; (e) dead cells. The progress may also be measured by a parameter representing accumulation per cell. method is rapid, avoids rinsing, fixation centrifugation applicable to microcultures. Multiparameter analysis which provides simultaneous measurements proliferation prove more sensitive assay histocompatibility than tests only (tritiated thymidine incorporation).
sagepub.com
sci-hub.se 参考文章(11)
Edward M. Johnson, Jonathan Karn, Vincent G. Allfrey, Early Nuclear Events in the Induction of Lymphocyte Proliferation by Mitogens: EFFECTS OF CONCANAVALIN A ON THE PHOSPHORYLATION AND DISTRIBUTION OF NON-HISTONE CHROMATIN PROTEINS Journal of Biological Chemistry. ,vol. 249, pp. 4990- 4999 ,(1974) , 10.1016/S0021-9258(19)42418-6
Bo Dupont, John A. Hansen, Edmond J. Yunis, Human mixed-lymphocyte culture reaction: genetics, specificity, and biological implications. Advances in Immunology. ,vol. 23, pp. 107- 202 ,(1976) , 10.1016/S0065-2776(08)60320-X
J.D. Braunstein, M.R. Melamed, T.K. Sharpless, J.A. Hansen, B. Dupont, R.A. Good, Quantitation of lymphocyte proliferative response to allogeneic cells and phytohemagglutinin by flow cytofluorometry Clinical Immunology and Immunopathology. ,vol. 5, pp. 326- 332 ,(1976) , 10.1016/0090-1229(76)90041-6
Fritz H. Bach, Marilyn L. Bach, Paul M. Sondel, Differential function of major histocompatibility complex antigens in T-lymphocyte activation. Nature. ,vol. 259, pp. 273- 281 ,(1976) , 10.1038/259273A0
F Traganos, Z Darzynkiewicz, T Sharpless, M R Melamed, Simultaneous staining of ribonucleic and deoxyribonucleic acids in unfixed cells using acridine orange in a flow cytofluorometric system. Journal of Histochemistry and Cytochemistry. ,vol. 25, pp. 46- 56 ,(1977) , 10.1177/25.1.64567
B. G. Pogo, V. G. Allfrey, A. E. Mirsky, RNA synthesis and histone acetylation during the course of gene activation in lymphocytes. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 55, pp. 805- 812 ,(1966) , 10.1073/PNAS.55.4.805
Z. Darżynkiewicz, L. Bolund, N.R. Ringertz, Nucleoprotein changes and initiation of RNA synthesis in PHA stimulated lymphocytes Experimental Cell Research. ,vol. 56, pp. 418- 424 ,(1969) , 10.1016/0014-4827(69)90033-0
ZBIGNIEW DARŻYNKIEWICZ, VIKTOR K. DOKOV, MAREK PIEŃKOWSKI, Dry mass of lymphocytes during transformation after stimulation by phytohaemagglutinin. Nature. ,vol. 214, pp. 1265- 1266 ,(1967) , 10.1038/2141265A0
ROBERTSON PARKMAN, FRED S. ROSEN, JOEL RAPPEPORT, BRUCE CAMITTA, RAPHAEL L. LEVEY, DAVID G. NATHAN, Detection of genetically determined histocompatibility antigen differences between HL-A identical and MLC nonreactive siblings. Transplantation. ,vol. 21, pp. 110- 116 ,(1976) , 10.1097/00007890-197602000-00004
Z. Darżynkiewicz, F. Traganos, T. Sharpless, M.R. Melamed, Thermal denaturation of DNA in situ as studied by acridine orange staining and automated cytofluorometry. Experimental Cell Research. ,vol. 90, pp. 411- 428 ,(1975) , 10.1016/0014-4827(75)90331-6