Arginine and Lysine Transporters Are Essential for Trypanosoma brucei.
作者： Christoph Mathieu , Juan P. Macêdo , Daniel Hürlimann , Corina Wirdnam , Alexander C. Haindrich
DOI: 10.1371/JOURNAL.PONE.0168775
关键词:
摘要: For Trypanosoma brucei arginine and lysine are essential amino acids therefore have to be imported from the host. Heterologous expression in Saccharomyces cerevisiae mutants identified cationic acid transporters among members of T. AAAP (amino acid/auxin permease) family. TbAAT5-3 showed high affinity uptake (Km 3.6 ± 0.4 μM) selectivity for L-arginine. L-arginine transport was reduced by a 10-times excess L-arginine, homo-arginine, canavanine or arginine-β-naphthylamide, while inhibitory only at 100-times excess, histidine ornithine did not reduce rates significantly. TbAAT16-1 is 4.3 0.5 highly selective L-lysine transporter compounds tested, thialysine were competing uptake. expressed both procyclic bloodstream form cMyc-tagged proteins indicate localization plasma membrane. RNAi-mediated down-regulation TbAAT5 TbAAT16 trypanosomes resulted growth arrest, demonstrating that TbAAT5-mediated TbAAT16-mediated brucei. Growth induced RNAi lines could partially rescued supplementing surplus lysine, respectively, addition less efficient. Single double additional low systems present
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