A nonconsensus octamer-recognition sequence (TAATGARAT-motif) identifies a novel DNA binding protein in human Merkel cell carcinoma cell lines.
作者： J. A. F. Thomson , J. H. Leonard , K. McGregor , R. A. Sturm , P. G. Parsons
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摘要: We have established a number of cell lines from Merkel carcinoma (MCC) the skin. In many respects these resemble those small lung cancer (SCLC) and it is difficult to differentiate between metastatic MCC SCLC on morphological or histochemical criteria. Both are thought be neuroendocrine tumours may express markers. octamer-DNA binding transcription factor brn-2 gene products N-Oct 3 5, which restricted neuroectodermal lineage. DNA studies using consensus octamer recognition site we found that 4 8 examined expressed in at least trace amounts compared with all proteins high levels. Moreover, were extended by high-affinity related degenerate TAATGARAT-motif. This identified novel protein subset lines. The was absent three lines, melanoma cells brain tissue. activity, term nuclear (MNF), shown specific competitive inhibition oligonucleotide sites not inhibited polyclonal antisera against Oct-1, Oct-2 Brn-2 proteins. serve as unique marker far SOLO involved regulating phenotype.
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