Itraconazole Alters the Stem Cell Characteristics of A549 and NCI-H460 Human Lung Cancer Cells by Suppressing Wnt Signaling

摘要: BACKGROUND Cancer stem cells (CSCs) behave as their malignant counterparts, but persist after treatment, and possess properties that allow them to interact with environment. Itraconazole, an antifungal agent, also has a role in suppressing tumor progression, its effects regulating cell stemness remain unclear. This study aimed evaluate the of itraconazole on A549 NCI-H460 human lung cancer vitro. MATERIAL AND METHODS BEAS-2B normal bronchial epithelial were cultured without itraconazole. Cell viability was evaluated. The expression markers, CD133, ATP binding cassette subfamily G member 2 (ABCG2), aldehyde dehydrogenase 1 (ALDH1), measured by Western blot quantitative real-time polymerase chain reaction (qRT-PCR). Sphere-forming evaluated RESULTS Itraconazole reduced molecules ABCG2, ALDH1 cells, numbers sphere-forming reduced. However, had little effect enhanced chemosensitivity cells. inhibited Wnt signaling. Re-activation signaling restored itraconazole-mediated inhibition stemness. CONCLUSIONS altered characteristics did not affect viability.