Modulation of anti-inflammatory response in lipopolysaccharide stimulated human THP-1 cell line and mouse model at gene expression level with indigenous putative probiotic lactobacilli.

摘要: The anti-inflammatory potential of eight indigenous probiotic Lactobacillus isolates was evaluated in vitro terms modulating the expression tumor necrosis factor-alpha (TNF-α) and interleukin-6 (IL-6) human acute monocytic leukemia (THP-1) cells under inflammatory conditions. Amongst these, plantarum Lp91 most potent strain as it evoked a significant (P < 0.001) down-regulation TNF-α by −1.45-fold relative to control THP-1 cells. However, IL-6 expression, all strains could up-regulate its considerably at different levels. Hence, based on TNF-α, selected for vivo study lipopolysaccharide (LPS)-induced mouse model look IL-6, monocyte chemotactic protein-1 (MCP-1), vascular cell adhesion molecule-1 (VCAM-1), intercellular molecule (ICAM-1) E-selectin aorta. In LPS challenged (2 h) mice group fed with 10 days, MCP-1, VCAM-1, ICAM-1 expressions were significantly down-regulated 3.10-, 10.02-, 4.22-, −3.14-, 2.28- 5.71-fold conclusion, serve candidate explore possible biotherapeutic agent against diseases including cardiovascular disease.