Calcineurin controls inositol 1,4,5-trisphosphate type 1 receptor expression in neurons.

摘要: In the central nervous system, release of Ca2+ from intracellular stores contributes to numerous functions, including neurotransmitter and long-term potentiation depression. We have investigated developmental profile regulation inositol 1,4,5-trisphosphate receptor (IP3R) ryanodine (RyR) in primary cultures cerebellar granule cells. The expression both types increases during development. Whereas type 1 IP3R appears be regulated by influx through L channels or N-methyl-d-aspartate (NMDA) receptors, RyR levels increase independently Ca2+. main target Ca2+-influx-regulating is calmodulin-dependent protein phosphatase calcineurin, because pharmacological blockade this abolishes expression. Although calcineurin has been shown regulate phosphorylation state IP3R, effect described here at transcriptional level mRNA changes parallel with levels. Thus, plays a dual role IP3R-mediated signaling: it regulates function dephosphorylation short-term time scale over more extended periods.