Molecular, biochemical and structural characterization of osmotin-like protein from black nightshade (Solanum nigrum)
作者： Pulugurtha Bharadwaja Kirti Sravan Kumar Jami , T. Swathi Anuradha , K. Vasavi Rama , Lalitha Guruprasad1
DOI: 10.1016/J.JPLPH.2006.01.006
关键词:
摘要: Summary A full-length 910 bp cDNA encoding osmotin-like protein with an open reading frame of 744 bp a 247 amino acids calculated molecular mass 26.8 kDa was cloned from Solanum nigrum (SniOLP). Phylogenetic analysis revealed the evolutionary conservation this among diverse taxa. The genomic DNA gel blot showed that SniOLP belongs to small multigene family and it organ-specific expression. Time-course studies expression upregulated by treatment various signaling molecules, osmotic oxidative stress inducers. Recombinant purified overexpressed Escherichia coli cells hyphal growth inhibition in Rhizoctonia batiticola Sclerotinia sclerotiorum but without any endo-β-1,3-glucanase activity. Model built homology modeling consists acidic cleft region is capable interacting carbohydrate components fungal cell walls. Analysis structure functional relationship carried out docking β-(1,3)-glucan onto on surface
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