Mechanisms of protection afforded by cyclooxygenase inhibitors to endothelial function against ischemic injury in rat isolated hearts.
作者： Jean-François Bouchard , Daniel Lamontagne
DOI: 10.1097/00005344-199911000-00019
关键词:
摘要: Summary: The aim of this study was to assess whether cyclooxygenase (COX) inhibitors protect the endothelial function against deleterious effect ischemia and reperfusion. Isolated rat hearts perfused under constant-flow conditions were exposed 30 min partial (flow, 1 ml/min) followed by 20 reperfusion, after which coronaries precontracted with U-46619, response endothelium-dependent vasodilator, serotonin (5-HT), compared that endothelium-independent sodium nitroprusside (SNP). In untreated hearts, diminished selectively 5-HT-induced vasodilation, sham (without ischemia). vasodilation SNP unaffected in all groups. Pretreatment 6-MNA, µM, a COX-2 inhibitor some activity on COX 1, diclofenac, µM (COX-1 -2), or 1-(7-carboxyheptyl) imidazole, 10 [thromboxane (TX) synthase inhibitor] but not indomethacin, inhibitor) preserved induced 5-HT ischemia. Enzyme immunoassays indicated decreased concentration TXB2 6-keto-PGF1[alpha] [stable metabolites TXA2 prostacyclin (PGI2), respectively] coronary effluent during Furthermore, indomethacin only one abolish PGE2 early No clear trend ventricular postischemic recovery could be observed between treated groups our experimental protocols. These data suggest that, conditions, 1-7-CHI, via reduction TX concentration. Disparities may due complete abolition reperfusion group.
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Jugdutt Bi, Basualdo Ca, Myocardial infarct expansion during indomethacin or ibuprofen therapy for symptomatic post infarction pericarditis. Influence of other pharmacologic agents during early remodelling. Canadian Journal of Cardiology. ,vol. 5, pp. 211- 221 ,(1989)
J. R. Bend, M. Karmazyn, Role of eicosanoids in the ischemic and reperfused myocardium. EXS. ,vol. 76, pp. 243- 262 ,(1996) , 10.1007/978-3-0348-8988-9_15
E.A. Meade, W.L. Smith, D.L. DeWitt, Differential inhibition of prostaglandin endoperoxide synthase (cyclooxygenase) isozymes by aspirin and other non-steroidal anti-inflammatory drugs. Journal of Biological Chemistry. ,vol. 268, pp. 6610- 6614 ,(1993) , 10.1016/S0021-9258(18)53294-4
K M Mullane, S Moncada, N Read, J A Salmon, Role of leukocytes in acute myocardial infarction in anesthetized dogs: relationship to myocardial salvage by anti-inflammatory drugs. Journal of Pharmacology and Experimental Therapeutics. ,vol. 228, pp. 510- 522 ,(1984)
Volker B. Fiedler, Role of Arachidonic Acid Metabolites in Cardiac Ischemia and Reperfusion Injury Pharmacotherapy. ,vol. 8, pp. 158- 168 ,(1988) , 10.1002/J.1875-9114.1988.TB04070.X
P. S Mankad, A. H Chester, M. H Yacoub, 5-Hydroxytryptamine mediates endothelium dependent coronary vasodilatation in the isolated rat heart by the release of nitric oxide. Cardiovascular Research. ,vol. 25, pp. 244- 248 ,(1991) , 10.1093/CVR/25.3.244
B I Jugdutt, G M Hutchins, B H Bulkley, B Pitt, L C Becker, Effect of indomethacin on collateral blood flow and infarct size in the conscious dog. Circulation. ,vol. 59, pp. 734- 743 ,(1979) , 10.1161/01.CIR.59.4.734
Kathleen M. O'Connor, Ted D. Friehling, Peter R. Kowey, The effect of thromboxane inhibition on vulnerability to ventricular fibrillation in the acute and chronic feline infarction models American Heart Journal. ,vol. 117, pp. 848- 853 ,(1989) , 10.1016/0002-8703(89)90622-4
Thomas J. Wargovich, Jawahar Mehta, Wilmer W. Nichols, Mary Beth Ward, Daniel Lawson, Daisy Franzini, C.Richard Conti, Reduction in myocardial neutrophil accumulation and infarct size following administration of thromboxane inhibitor U-63,557A. American Heart Journal. ,vol. 114, pp. 1078- 1085 ,(1987) , 10.1016/0002-8703(87)90182-7
G Ahumada, Synthesis of prostaglandins by cultured rat heart myocytes and cardiac mesenchymal cells Journal of Molecular and Cellular Cardiology. ,vol. 12, pp. 685- 700 ,(1980) , 10.1016/0022-2828(80)90099-1