A Novel Role for MiR-520a-3p in Regulating EGFR Expression in Colorectal Cancer.

作者: Rui Zhang , Rui Liu , Chang Liu , Yahan Niu , Jianguo Zhang

DOI: 10.1159/000479397

关键词:

摘要: Background/Aims: MicroRNAs (miRNAs) have been consistently demonstrated to be involved in colorectal cancer as either tumour oncogenes or suppressors. However, the detailed role of miR-520a-3p remains poorly understood. Methods: Quantitative RT-PCR and western blotting assays were used measure EGFR expression levels tissues, respectively. Luciferase reporter assay was employed validate direct targeting by miR-520a-3p. Cell migration, apoptosis cell cycle performed analyse biological functions cells. In vivo experiment effects on growth xenografts mice. Results: this study, we found that most likely target 3’-UTR, which experimentally validated. addition, investigated inhibition both vitro could suppress promote apoptosis, lead arrest at G0/G1 phase, decelerate xenograft mice, potentially EGFR. Conclusions: This study highlights a suppressor for via regulation expression. Thus, may novel molecular therapeutic cancer.

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