Standardized reagents and protocols for engineering zinc finger nucleases by modular assembly.

作者: David A Wright , Stacey Thibodeau-Beganny , Jeffry D Sander , Ronnie J Winfrey , Andrew S Hirsh

DOI: 10.1038/NPROT.2006.259

关键词:

摘要: … In vitro SELEX was used to characterize the DNA-binding specificities of most of these fingers 22 . These modules were generated by altering fingers from the three-finger Sp1 zinc …

参考文章(59)
Stacey A. Thibodeau, Rui Fang, J. Keith Joung, High-throughput β-galactosidase assay for bacterial cell-based reporter systems BioTechniques. ,vol. 36, pp. 410- 415 ,(2004) , 10.2144/04363BM07
Jeffrey H. Miller, A short course in bacterial genetics ,(1992)
Mark Isalan, Aaron Klug, Yen Choo, A rapid, generally applicable method to engineer zinc fingers illustrated by targeting the HIV-1 promoter Nature Biotechnology. ,vol. 19, pp. 656- 660 ,(2001) , 10.1038/90264
Scot A. Wolfe, Lena Nekludova, Carl O. Pabo, DNA recognition by Cys2His2 zinc finger proteins. Annual Review of Biophysics and Biomolecular Structure. ,vol. 29, pp. 183- 212 ,(2000) , 10.1146/ANNUREV.BIOPHYS.29.1.183
Kent G Golic, Dana Carroll, Mary Golic, Marina Bibikova, Targeted chromosomal cleavage and mutagenesis in Drosophila using zinc-finger nucleases. Genetics. ,vol. 161, pp. 1169- 1175 ,(2002) , 10.1093/GENETICS/161.3.1169
Ryuk-Jun Kwon, Seong Keun Kim, Seung-Il Lee, Su-Jeong Hwang, Gyun Min Lee, Jin-Soo Kim, Wongi Seol, Artificial transcription factors increase production of recombinant antibodies in Chinese hamster ovary cells. Biotechnology Letters. ,vol. 28, pp. 9- 15 ,(2006) , 10.1007/S10529-005-4680-7
Mala Mani, Karthikeyan Kandavelou, Fei Jamie Dy, Sundar Durai, Srinivasan Chandrasegaran, Design, engineering, and characterization of zinc finger nucleases Biochemical and Biophysical Research Communications. ,vol. 335, pp. 447- 457 ,(2005) , 10.1016/J.BBRC.2005.07.089
Kyung-Soon Park, Dong-ki Lee, Horim Lee, Yangsoon Lee, Young-Soon Jang, Yong Ha Kim, Hyo-Young Yang, Sung-Il Lee, Wongi Seol, Jin-Soo Kim, Phenotypic alteration of eukaryotic cells using randomized libraries of artificial transcription factors. Nature Biotechnology. ,vol. 21, pp. 1208- 1214 ,(2003) , 10.1038/NBT868
Kwang-Hee Bae, Young Do Kwon, Hyun-Chul Shin, Moon-Sun Hwang, Eun-Hyun Ryu, Kyung-Soon Park, Hyo-Young Yang, Dong-ki Lee, Yangsoon Lee, Jinwoo Park, Heung Sun Kwon, Hyun-Won Kim, Byung-Il Yeh, Hyean-Woo Lee, Soon Hyung Sohn, Joonho Yoon, Wongi Seol, Jin-Soo Kim, None, Human zinc fingers as building blocks in the construction of artificial transcription factors. Nature Biotechnology. ,vol. 21, pp. 275- 280 ,(2003) , 10.1038/NBT796