The concentration of 5-phosphoribosyl 1-pyrophosphate in monolayer tumor cells and the effect of various pyrimidine antimetabolites.
作者： G.J. Peters , E. Laurensse , A. Leyva , H.M. Pinedo
DOI: 10.1016/0020-711X(85)90091-6
关键词:
摘要: 5-Phosphoribosyl 1-pyrophosphate (PRPP) was determined in several murine and human cancer cell lines grown monolayer, harvested by trypsinization. For all a large variation the PRPP concentration (5-1300 pmol/ 10(6) cells found. A 1-hr incubation Dullbecco's medium reduced concentration. After this highest found B16 melanoma line (about 200 pmol/10(6) cells). The IGR3 M5 colon carcinoma WiDr contained about 100 cells. preincubation of these suspensions were used to study effect antimetabolites on 2-hr with 1mM N-(phosphonacetyl)-L-aspartate (PALA) increased only cells, whereas methotrexate caused an increase lines. When 5-fluorouracil (5FU) added, no significant decrease any line. Addition 5FU after PALA resulted lower B16, prodrug, 5-fluoro-5' deoxyuridine altered when it added PALA. activity metabolizing enzyme orotate phosphoribosyl transferase comparable but much
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