Benzo(a)pyrene and 7,12-dimethylbenz(a)anthrecene differentially affect bone marrow cells of the lymphoid and myeloid lineages

摘要: Polycyclic aromatic hydrocarbons (PAHs) are common environmental contaminants that carcinogenic and immunosuppressive. Benzo(a)pyrene (BP) 7,12-dimethylbenz(a)anthracene (DMBA) two prototypic PAHs known to impair the cell-mediated humoral immune responses. We have previously shown that, in C57BL/6J mice, total bone marrow (BM) cellularity decreased two-fold following intraperitoneal DMBA treatment but not BP treatment. Here, we used flow cytometry demonstrate differentially alter lymphoid myeloid lineages. Following treatment, pro/pre B-lymphocytes (B220(lo)/IgM(-)) immature (B220(lo)/IgM(+)) significantly decreased, while mature (B220(hi)/IgM(+)) remained unaffected. In contrast, B-lymphocytes, did affect or B-lymphocytes. The Gr-1(+) cells of lineage were depleted 50% only minimally Interestingly, monocytes (7/4(+)1A8(lo)) neutrophils (7/4(+)1A8(hi)) within this population affected by these PAHs. Monocytes whereas increased Although TNFalpha CYP1B1 implicated as essential mediators hypocellularity, similar induction mRNA BM suggests they limiting factors mediating different effects Given amounts reach when administered intraperitoneally, their differential on lineages probably stem from differences reactive metabolites such PAH quinones PAH-dihydrodiol-epoxides.