Detection of trichothecene producing Fusarium spp. by PCR: adaptation, validation and application to fast food
作者： Martina Barchitta , Salvatore Sciacca , Ludwig Niessen , Margherita Ferrante , Antonella Agodi
DOI: 10.2427/5990
关键词:
摘要: Background . Food contamination by trichothecene mycotoxins is considered to be an emerging public health problem. The aim of this study was validate a rapid sonification protocol, previously set up for cereal Fusarium DNA extraction from fast food samples, produced centre research and development in the industry Catania, Sicily, it diagnostic PCR assay targeted at tri5, key gene biosynthesis. Methods reference spp. strains samples prepared, setting protocol adapted using some modifications based on method recently described. Validation experiments were performed: serial dilution extracted fungal cultures added then extracted. Specific primer pairs used detect F. graminearum culmorum species-specific assays as well trichothecene-producing groupspecific system. Results All genomic DNA-spiked still it’s original condition resulted correct amplification. detection limit 1 x 10-4 μg DNA. tested gave highly consistent results repeatability assays, thus demonstrating within-lab within/between-day precision method. Conclusions Information epidemiology producing through chain identification most frequently contaminated components are essential order develop effective strategies minimising consumer exposure trichothecenes. Key words: Fusarium, food, trichothecenes,
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