Development and characterization of pediatric osteosarcoma xenografts.

摘要: Abstract Of 33 surgical specimens of osteosarcoma obtained from 24 patients, eight were established as transplantable tumor lines in immune-deprived CBA/CaJ inbred mice. Each line retained the histological characteristics corresponding primary and produced human lactate dehydrogenase isozymes. Volume doubling times, which ranged a mean 12.3 ± 5.6 to 39.3 9.8 days, stable for individual over multiple passages. Flow cytometric analysis indicated similar cellular DNA content values tumors xenograft lines; presence two separate stem lines, original tumors, was observed laboratory models. Comparison methods immune deprivation that thymectomy, whole-body irradiation, bone marrow reconstitution associated with higher rate successful engraftment than 1-β-d-arabinofuranosylcytosine treatment, irradiation. Bone marrow-reconstituted mice also showed less variability volume time. We conclude can be heterotransplanted into relatively high success xenografts retain features characteristic origin. The availability these models should prove useful development new therapeutic regimens understanding biology osteosarcoma.