The adsorption of preferential binding peptides to apatite-based materials

摘要: The objective of this work was to identify peptide sequences with high affinity bone-like mineral (BLM) provide alternative design methods for functional bone regeneration peptides. Adsorption preferential binding on four apatite-based substrates [BLM and three sintered apatite disks pressed from powders containing 0% CO(3)(2-) (HA), 5.6% (CA5), 10.5% (CA10)] varied compositions morphologies investigated. A combination phage display, ELISA, computational modeling used elucidate 12-mer APWHLSSQYSRT (A), STLPIPHEFSRE (S), VTKHLNQISQSY (V), 243 candidates adsorption BLM HA. Overall, peptides S V have a significantly higher the materials in comparison (for vs. A, p=0.001, CA5 p<0.001, CA10 HA p=0.038; p=0.006, p=0.033, p=0.029). FT-IR analysis displayed carbonate levels dropped approximately 1.1-2.2% after sintering, whereas SEM imaging possess distinct morphologies. results normalized surface area indicate that small changes percentage at similar morphological scale did not enough incorporation show statistical differences adsorption. Because identified (S V) apatite, their use can now be investigated dentin tissue engineering, tendon ligament repair, enamel formation.