Synergistic in vitro photodynamic antimicrobial activity of methylene blue and chitosan against Helicobacter pylori 26695
作者： Sung Sook Choi , Hae Kyung Lee , Hiun Suk Chae
DOI: 10.1016/J.PDPDT.2014.08.005
关键词:
摘要: Summary Background Photodynamic therapy (PDT) is a method for killing cells (bacterial, fungal and cancer cells) or virus using photosensitizers (PS) light of various wavelengths. In vitro PDT endoscopic against H. pylori was effective at concentration 0.2 mg/mL MB. The purpose this study to increase the effect photodynamic modality by addition chitosan Methods bactericidal measured counting viable after PDT. degree damage DNA confirmed alkaline gel electrophoresis. Cellular demonstrated ethidium bromide monoazide–quantitative polymerase chain reaction (EMA–qPCR). Results groups treated with either 0.04 mg/mL MB alone 0.02 mg/mL 15 min, were decreased approximately tenfold. group light, showed more activity than treatment. By 0.05% pre-treatment followed irradiation, 107-fold. caused as electrophoresis greater in plus chitosan-treated control MB-treated groups. EMA–qPCR also group. Conclusion effects increased photosensitizer treatment, peculiarly before irradiation.
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