Identification of proteomic signatures associated with depression and psychotic depression in post-mortem brains from major depression patients

摘要: Major depressive disorder (MDD) is a leading cause of disability worldwide and results tragically in the loss almost one million lives Western societies every year. This due to poor understanding disease pathophysiology lack empirical medical tests for accurate diagnosis or guiding antidepressant treatment strategies. Here, we have used shotgun proteomics analysis post-mortem dorsolateral prefrontal cortex brain tissue from 24 MDD patients 12 matched controls. Brain proteomes were pre-fractionated by gel electrophoresis further analyzed data-independent label-free liquid chromatography-mass spectrometry. led identification distinct proteome fingerprints between control subjects. Some these differences validated blot selected reaction monitoring mass included proteins associated with energy metabolism synaptic function also found changes histidine triad nucleotide-binding protein 1 (HINT1), which has been implicated recently regulation mood behavior. We differential profiles (n=11) without (n=12) psychosis. Interestingly, psychosis fingerprint showed marked overlap seen schizophrenia patients. These findings suggest that it may be possible contribute distinguishing different subtypes based on proteomic profiles.