Effect of seed sludge and operation conditions on performance and archaeal community structure of low-temperature anaerobic solvent-degrading bioreactors.
作者： Anne-Marie Enright , Vincent McGrath , Darryl Gill , Gavin Collins , Vincent O’Flaherty
DOI: 10.1016/J.SYAPM.2008.10.003
关键词:
摘要: Abstract Two laboratory-scale expanded granular sludge bed (EGSB) anaerobic bioreactors (R1 and R2) were inoculated with biomass from different mesophilic (37 °C) treatment plants, used for the of an organic solvent-based wastewater at 9–14 °C applied loading rates (OLRs) 1.2–3.6 kg chemical oxygen demand (COD) m −3 d −1 . Replicated performance was observed 10–14 °C, which suggested feasibility process pilot-scale. Stable efficient COD removal, along high methane productivity, demonstrated 9 °C OLR 2.4 kg COD m Clonal libraries fluorescence in situ hybridization (FISH) indicated that seed sludges dominated (>60%) by acetoclastic Methanosaeta -like organisms. Specific methanogenic activity (SMA) profiles shifts physiological R1 R2 biomass, including development psychrotolerant activity. Acetoclastic methanogenesis represented primary route production R2, is contrast several previous reports low-temperature bioreactor trials. A reduction abundance clones (R2) , detection hydrogenotrophic species, coincided altered granule (sludge) morphology SMA after prolonged operation 9 °C.
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