Identity and diversity of archaeal communities during anaerobic co-digestion of chicken feathers and other animal wastes

作者: Yun Xia , Daniel I. Massé , Tim A. McAllister , Yunhong Kong , Robert Seviour

DOI: 10.1016/J.BIORTECH.2012.01.107

关键词: Food scienceDigestionMethanomicrobialesManureMethanosarcinalesMicrobiologyBiologyArchaeaMethanobacteriales16S ribosomal RNAFeather

摘要: Abstract Digestion of raw feathers in anaerobic digesters inoculated with adapted swine manure, slaughterhouse sludge or dairy manure was investigated using twelve 42-L at 25 °C. After 120 days 74%, 49% and 40% added were converted to methane respectively. 16S rRNA gene clone library analyses identified twenty-one operational taxonomic units containing sequences from 5 genera, families 2 phyla members the Archaea 158 sequenced clones. Fluorescence situ hybridization revealed that methanogens Methanomicrobiales, Methanosarcinales Methanobacteriales constituted a major fraction (>78%) these Archaea. A high correlation seen between distribution functional archaeal groups NH3–N levels digester mixed liquors. The compositions communities fed different substrates statistically significantly (P

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