Expression and regulation of nuclear retinoic acid receptors in human lymphoid cells.

摘要: Retinoids are known to play an important role in cellular growth and differentiation more recently the immune response. Our laboratory has previously shown that all-trans-retinoic acid (atRA) augments immunoglobulin synthesis of cord blood mononuclear cells by enhancing certain cytokines. Transcriptional regulatory elements, retinoic nuclear receptors (RAR), could mediate RA-induced regulation genes, e.g., cytokines whose products involved pathways synthesis. Although much is about RAR various animal species tissues, little expression its isotypes human lymphoid cells. In this study, we examined (RAR-α, RAR-β, RAR-γ) their respective isoforms T- B-lymphoid using a quantitative RT-PCR assay. RAR-α1 -γ1 were both constitutively expressed did not change with addition atRA B-cell lines or adenoidal T B lymphocytes. contrast, RAR-β2 was detected. The cell culture produced marked increase amounts mRNA (2.2- 41-fold). As isoform, increased RAR-α2 levels (3.4- 17-fold), but only EBV-transformed RAR-β1 -β3 undetectable inducible atRA. RAR-γ2 at very low results suggest expressions -β2 highly controlled Differences may be factor modulation cytokine production augmentation Ig