MALDI mass spectrometry based molecular phenotyping of CNS glial cells for prediction in mammalian brain tissue

摘要: The development of powerful analytical techniques for specific molecular characterization neural cell types is central relevance in neuroscience research elucidating cellular functions the nervous system (CNS). This study examines use differential protein expression profiling mammalian cells using direct analysis by means matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS). MALDI-MS rapid, sensitive, robust, and large biomolecules complex matrices. Here, we describe a newly developed straightforward methodology rodent CNS glial MALDI-MS-based intact (ICMS). phenotyping approach enables monitoring growth stages, (stem) differentiation, as well probing responses towards different stimulations. Glial were separated into pure astroglial, microglial, oligodendroglial cultures. suspensions then analyzed directly MALDI-TOF-MS, resulting characteristic spectra profiles that discriminated principal component analysis. Complementary proteomic experiments revealed identity these signature proteins predominantly expressed types, including histone H4 oligodendrocytes S100-A10 astrocytes. MALDI imaging MS was performed, masses employed tracers prediction astroglial localization brain tissue. type distributions tissue validated immunohistochemistry. ICMS neuroglia simple discrimination with specificity.