Importance of RNA stabilization: evaluation of ansB, ggt, and rpoA transcripts in microaerophilic Campylobacter jejuni 81-176
作者： Heidi Hyytiäinen , Pekka Juntunen , Nina Akrenius , Marja-Liisa Hänninen
DOI: 10.1007/S00203-012-0820-3
关键词:
摘要: A subset of food-borne Campylobacter jejuni strains utilizes amino acids asparagine and glutamine as carbon sources that may enhance the ability this microaerophilic pathogen to colonize specific tissues. In study, we analyzed transcript sizes ansB ggt genes encoding periplasmic asparaginase γ-glutamyltranspeptidase in C. 81-176, respectively, compared expression level mRNAs at different time points during growth vitro. addition, included housekeeping rpoA gene, α-subunit DNA-directed RNA polymerase, monitor sample processing it has been described a stable reference gene studies jejuni. Our results revealed both were expressed end logarithmic phase their corresponding monocistronic not affected by steps. contrast, polycistronic operon containing highly induced earlier stage clearly differentially responding external factors cell harvesting step.
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