Cyanide and Carbon Monoxide Ligand Formation in Hydrogenase Biosynthesis

摘要: Carbon monoxide (CO) and cyanide (CN–) can act as potent inhibitors of enzymes necessary for primary biochemical processes, however they also play important roles in biological systems. Well-studied cases include CN– biosynthesis plants to a defense against herbivores pathogens, certain species bacteria remove excess glycine, CO by microbes energy metabolism the Wood–Ljungdahl pathway. The utilization essential metal ligands biology is even more limited, with only known examples being at active sites hydrogenase enzymes. This class catalyzes reversible oxidation hydrogen, reaction that appears be entirely dependent on presence and/or ligands. To date, synthetic mimicsof have not reproduced hydrogen production rates observed some hydrogenases; it thus considerable interest understand how has solved intriguing problem biosynthesizing efficient catalysts. Of discussed herein, recent advances [FeFe]-hydrogenase family provided insights into synthesis its site (H-cluster). Biosynthesis complex requires three iron–sulfur cluster-containing maturation proteins, where two radical S-adenosylmethionine (AdoMet) (HydE HydG) other GTPase (HydF). In this review, genesis mechanisms will assessed specific focus maturation.