Detection of EnterotoxigenicStaphylococcus aureusin Raw Milk and Dairy Products by Multiplex PCR
作者： Ali Gücükoğlu , T. Onur Kevenk , Tolga Uyanik , Özgür Çadirci , Göknur Terzi
DOI: 10.1111/J.1750-3841.2012.02954.X
关键词:
摘要: Abstract: The aim of this study was to investigate the prevalence enterotoxigenic Staphylococcus aureus in 122 samples, including 60 raw milk, 32 white cheese, 10 kashar butter, and ice cream samples obtained from Samsun province, Turkey. In study, S. detected 64 milk (45/60; 75%), cheese (12/32; 37.5%), (3/10; 30%), butter (1/10; 10%) samples. A total 81 isolates were identified as by PCR with presence 16S rRNA nuc genes. genes encoding staphylococcal enterotoxins (SEs) SEA, SEB, SEC, SED multiplex PCR. According analysis, seven (7/51; 13.7%) enterotoxigenic; five them produced SEA (5/7; 71.4%), one SEB (1/7; 14.2%), SEA+SEB 14.2%). Four (4/21; 19%) (1/4; 25%), SEC SEA+SED 25%) toxins. Two (2/4; 50%) found be (1/2; other 50%). One isolate showed character (SEB, 1/1; 100%). products potentially hazardous public health because fact that levels contamination higher than 105–106 cfu/g ml 39% (25/64, 17 7 1 butter) analyzed
sci-hub.se 参考文章(29)
J. McLAUCHLIN, G. L. NARAYANAN, V. MITHANI, G. O'NEILL, The detection of enterotoxins and toxic shock syndrome toxin genes in Staphylococcus aureus by polymerase chain reaction. Journal of Food Protection. ,vol. 63, pp. 479- 488 ,(2000) , 10.4315/0362-028X-63.4.479
Yves Le Loir, Michel Gautier, Florence Baron, Staphylococcus aureus and food poisoning. Genetics and Molecular Research. ,vol. 2, pp. 63- 76 ,(2003)
L. Necidová, Z. Šťástková, M. Pospíšilová, B. Janštová, J. Strejček, M. Dušková, R. Karpíšková, Influence of soft cheese technology on the growth and enterotoxin production of Staphylococcus aureus Czech Journal of Food Sciences. ,vol. 27, pp. 127- 133 ,(2018) , 10.17221/131/2008-CJFS
S Unal, J Hoskins, J E Flokowitsch, C Y Wu, D A Preston, P L Skatrud, Detection of methicillin-resistant staphylococci by using the polymerase chain reaction. Journal of Clinical Microbiology. ,vol. 30, pp. 1685- 1691 ,(1992) , 10.1128/JCM.30.7.1685-1691.1992
Paul M. Orwin, J. Ross Fitzgerald, Donald Y. M. Leung, Juan A. Gutierrez, Gregory A. Bohach, Patrick M. Schlievert, Characterization of Staphylococcus aureus Enterotoxin L Infection and Immunity. ,vol. 71, pp. 2916- 2919 ,(2003) , 10.1128/IAI.71.5.2916-2919.2003
C. Letertre, S. Perelle, F. Dilasser, P. Fach, Identification of a new putative enterotoxin SEU encoded by the egc cluster of Staphylococcus aureus. Journal of Applied Microbiology. ,vol. 95, pp. 38- 43 ,(2003) , 10.1046/J.1365-2672.2003.01957.X
S. Morandi, M. Brasca, R. Lodi, P. Cremonesi, B. Castiglioni, Detection of classical enterotoxins and identification of enterotoxin genes in Staphylococcus aureus from milk and dairy products. Veterinary Microbiology. ,vol. 124, pp. 66- 72 ,(2007) , 10.1016/J.VETMIC.2007.03.014
H. Yeşim Can, T. Haluk Çelik, Detection of enterotoxigenic and antimicrobial resistant S. aureus in Turkish cheeses Food Control. ,vol. 24, pp. 100- 103 ,(2012) , 10.1016/J.FOODCONT.2011.09.009
C.H. Doan, P.M. Davidson, Growth and Production of Enterotoxin A by Staphylococcus aureus on "Home-style" French Fries Journal of Food Science. ,vol. 64, pp. 913- 917 ,(1999) , 10.1111/J.1365-2621.1999.TB15939.X
H.J. Jørgensen, T. Mørk, H.R. Høgåsen, L.M. Rørvik, Enterotoxigenic Staphylococcus aureus in bulk milk in Norway Journal of Applied Microbiology. ,vol. 99, pp. 158- 166 ,(2005) , 10.1111/J.1365-2672.2005.02569.X