Immunohistochemical Detection of EGFR in Paraffin-embedded Tumor Tissues: Variation in Staining Intensity Due to Choice of Fixative and Storage Time of Tissue Sections
作者： Derek Atkins , Karl-August Reiffen , Conny Lund Tegtmeier , Henrik Winther , Marcellus S. Bonato
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摘要: The epidermal growth factor receptor (EGFR) is highly expressed in a variety of solid malignant tumors and its expression has been correlated with disease progression poor survival. With the advent targeted therapies, especially IMC-C225 (Cetuximab), monoclonal antibody (MAb) directed against EGFR, there an increasing interest immunohistochemistry (IHC)-based EGFR screening methods using paraffin-embedded tumor specimens to select cancer patients eligible for treatment Cetuximab. EGFRpharmDX kit, complete assay demonstration now available. Because no information about preservation under various conditions fixation available, we performed prospective study on panel commonly used fixatives determine optimal tissue protocols. stability epitope cut sections stored period up 24 month was also tested material originating from head neck cancer, non-small-cell lung c...
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