Isolating Hydra Mutants by Sexual Inbreeding

摘要: The genetic approach for the study of hydra development promises to be a powerful one. This approach, however, has been attempted only in few cases (Lenhoff, 1965; Moore and Campbell, 1973; Sugiyama Fujisawa, 1977 a), presumably because ( 1) hydra's sexual reproduction is sporadic, slow, difficult control; (2) design efficient screening procedures mutants appears at present not practical. To isolate mutants, we use inbred strains obtained from field rather than attempting induce mutagenesis cultures animals grown laboratory long periods. that are heterozygous recessive mutant genes made homozygous by reproducing sexually laboratory. resultant propagated asexual breeding as clones. In restricted ourselves searching developmental can discovered simply examining many visually. procedure designed Hydra magnipapillata,