Suramin treatment of human glioma xenografts; effects on tumor vasculature and oxygenation status.

摘要: In this study the effect of suramin on tumor growth, vascularity and oxygenation a human glioma xenografted in nude mouse was examined. Vascular parameters status xenografts were determined immunohistochemically frozen sections tumors, using hypoxia marker pimonidazole-hydrochloride to detect hypoxic areas. Tumor vessels these stained by an endothelial cell perfusion visualized administration Hoechst 333342 before harvesting tumors. The vascular quantified with image analysis system. results show that growth reduced considerably after treatment. This suppression accompanied marked changes architecture. Although total area perfused fraction remained unchanged treatment, density increased, indicating more but smaller vessel structures had developed during therapy. These also homogeneously spread over area. Control tumors showed extensive areas while treated mostly disappeared. probably due higher distributed contributing increased tumor. observations suggest therapy can result not only which may have important consequences for sensitivity other therapies such as radiation