Molecular epidemiology of human oral Chagas disease outbreaks in Colombia.

摘要: Background Trypanosoma cruzi, the causative agent of Chagas disease, displays significant genetic variability revealed by six Discrete Typing Units (TcI-TcVI). In this pathology, oral transmission represents an emerging epidemiological scenario where different outbreaks associated to food/beverages consumption have been reported in Argentina, Bolivia, Brazil, Ecuador and Venezuela. Colombia, human corroborating importance route. Molecular epidemiology is barely known observing incrimination TcI, TcII, TcIV TcV genotypes. Methodology Principal Findings High-throughput molecular characterization was conducted performing MLMT (Multilocus Microsatellite Typing) mtMLST (mitochondrial Multilocus Sequence strategies on 50 clones from ten isolates. Results allowed occurrence mixed infection distinct TcI genotypes. Thus, a majority specific mitochondrial haplotypes allelic multilocus genotypes sylvatic cycle were detected dataset with foreseen presence domestic transmission. Conclusions These findings suggest occurred Colombia. We observed patterns super-infection and/or co-infection tailored association severe forms myocarditis acute phase disease. The dynamics route based evidence unraveled clinical biological implications are discussed.